Nosocomial Isolates and Their Drug Resistant Pattern in ICU Patients at National Institute of Neurological and Allied Sciences, Nepal

نویسندگان

  • Pashupati Bhandari
  • Ganesh Thapa
  • Bharat Mani Pokhrel
  • Dwij Raj Bhatta
  • Upendra Devkota
چکیده

Multidrug resistant organisms are increasing day by day and the cause is poorly known. This study was carried out from June 2011 to May 2012 at National Institute of Neurological and Allied Sciences Kathmandu, Nepal, with a view to determining drug resistant pathogens along with detection of extended spectrum β-lactamase (ESBL), AmpC β-lactamase (ABL), and metallo-β-lactamase (MBL) producing bacteria causing infection to ICU patients. A standard methodology was used to achieve these objectives as per recommendation of American Society for Microbiology. ESBL was detected by combined disc assay using cefotaxime and cefotaxime clavulanic acid, ABL by inhibitor based method using cefoxitin and phenylboronic acid, and MBL by imipenem-EDTA combined disk method. Two hundred and ninety-four different clinical samples such as tracheal aspirates, urine, pus, swabs, catheter tips, and blood were processed during the study. Most common bacteria were Acinetobacter spp. Of the total 58 Acinetobacter spp., 46 (79%) were MDR, and 27% were positive for ABL and 12% were for MBL. Of the 32 cases of Staphylococcus aureus, 18 (56%) were MDR. Findings of this study warrant routine β-lactamase testing in clinical isolates.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Prevalence of MDR, XDR and PDR Phenotypes among Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients in Mazandaran Province, Iran

Background and purpose: Pseudomonas aeruginosa is an opportunistic pathogen and the second leading cause of nosocomial infections, especially in the intensive care unit (ICU), and is responsible for high mortality rates. The aim of this study was to determine the pattern of antibiotic susceptibility and prevalence of multiple drug resistance strains of P. aeruginosa isolated from hospitalized p...

متن کامل

Drug Resistance of Pseudomonas aeruginosa and Enterobacter cloacae Isolated from ICU, Babol, Northern Iran

Multidrug resistant (MDR) bacteria are spread through the world which causes nosocomial infections, especially in Intensive Care Unit (ICU). This study aimed to investigate resistance pattern of Pseudomonas aeruginosa and Enterobacter cloacae isolated from patients in ICU. During 2011-2012, 30 isolates for each P. aeruginosa and E. cloacae were collected from patients who acquired nosocomial in...

متن کامل

Identification and antifungal susceptibility pattern of Candida species isolated from patients with nosocomial candiduria

Introduction: Nosocomial candiduria could be due to cystitis, pyelonephritis, or fungusball in the urinary tract system. Several reports have shown candidemia and upper urinarytract involvement as the complications of candiduria. The aim of this study was to assessnosocomial candiduria; identify Candida isolates and determine their drug susceptibilitypattern. Materials and Methods: Urine sample...

متن کامل

Antimicrobial Resistance Pattern of Acinetobacter baumannii Strains Isolated from Intensive Care Unit Patients

ABSTRACT             Background and Objectives: Acinetobacter baumannii is an opportunistic pathogen that affects different groups of people, especially intensive care unit (ICU) patients. The prevalence of infections caused by this bacterium is very high. Today, prevalence of infections caused by multidrug-resistant (MDR) and extreme-drug resistant (XDR) strains is increasing. This study aime...

متن کامل

A Survey of Frequency and pattern of Methicillin-Resistant Staphylococcu s aureus Isolated from nosocomial infections

Abstract Background and Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen involved in nosocomial infections. Because of increasing antibiotic resistance of these strains, treatment of these infections has become very difficult. This study aimed to determine the frequency and drug resistance pattern of MRSA isolates from nosocomial infections in hospi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2015  شماره 

صفحات  -

تاریخ انتشار 2015